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Journal: Epigenetics
Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage
doi: 10.1080/15592294.2024.2337087
Figure Lengend Snippet: Differentially methylated CpG sites between healthy controls and RSM patients. (a) The purity of the isolated decidual macrophages was detected by FACS. The cells that were positive for CD45 and CD14 were considered decidual macrophages. CD45 + CD14 + cells were also positive for CD68, a pan-macrophage marker. (b) Principal component analysis clearly showed separation between normal macrophages (red square, n = 3) and RSM macrophages (yellow square, n = 3). (c) Heatmap generated from clustering- analysis of microarray data illustrating differentially methylated DNA sites in the RSM patient group relative to the control group. Blue and red represent hypomethylation and hypermethylation, respectively, whereas white indicates no change in methylation relative to the control. Each row represents the beta value of a differentially methylated CpG site and each column represents one sample. (d) Pathways enriched in differentially methylated genes were determined using the GO database.
Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the
Techniques: Methylation, Isolation, Marker, Generated, Microarray
Journal: Epigenetics
Article Title: Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage
doi: 10.1080/15592294.2024.2337087
Figure Lengend Snippet: Validation of the microarray results via qRT – PCR. the data are presented as the means ± SDs and were analysed by two-sided unpaired Student’s t tests. ** p < 0.01.
Article Snippet: To assess differences in gene expression in decidual macrophages between RSM patients and control individuals, transcriptome profiles were obtained from the two groups of patients using the
Techniques: Microarray, Quantitative RT-PCR